Project Description
The Single and Dual Epithelial Voltage Clamp Systems are designed to be used with a corresponding Ussing Chamber for studies of epithelial transport and the electrical properties of tissues. Measurements include transepithelial voltage, short circuit current and membrane resistance.
Each System includes:
- PowerLab 8/35 and LabChart Pro software
- 19 Inch Rack Adapter
- The Epithelial Voltage Clamp (120 V) OR the Dual Channel Epithelial Voltage Clamp (50 V), depending on the system purchased.
Self-Contained Ussing Chamber Accessories purchased separately:
- Ussing Chamber Base Assembly (Single or Dual)
- Culture Cup Inserts for adaptors (Snapwell – 12 mm, Millicell – 12 mm, Transwell – 6.5 mm)
- Tissue Inserts with O-Ring (3.8, 6.0, 9.0, 12.0 and 13.5 mm)
- Tissue Inserts, Round with 5 Pins (3.8, 6.0, 9.0, 12.0 and 13.5 mm)
- Tissue Inserts, Slotted with 6 Pins (1.6 x 7.0, 2.4 x 7.0 and 3.6 x 7.0 mm)
Features
- Differential voltage recordings are made with very high common mode rejection (CMR) providing accurate measurements free from the effects of common mode potential changes of a noisy environment.
- Membrane Resistance Measurement Circuit using a low frequency 2 Hz bipolar signal to avoid polarization of the membrane (ideal for monolayers).
- Three Clamp Speeds selection provide optimum recording conditions for a variety of applications, such as Fast mode (as fast as 10 µsec) for preparations with low access resistance (small tissues or monolayers) and Medium or Slow modes for stable, oscillation free clamping in larger tissues.
- Operating modes include voltage clamp, current clamp, voltmeter, and resistance.
- Independent Voltage and Current Commands.
- Internal DC Command (Hold) control for both Voltage clamp and Current clamp modes.
- The clamp can be operated by an external programmer, lab timer or computer – logic control of clamp mode and clamp command levels is possible as well as simultaneous mixing of external linear commands.
- Watertight Headstage with Model Membrane
Product Codes
PL3508B78-V
Dual Channel Epithelial Voltage Clamp System
PL3508B77-V
Single Channel Epithelial Voltage Clamp System
Technical
Ordering Information
When ordering please specify the following: Mains Power (V) = 115 for 110-115 V or 220 for 220-240 V.
Specifications
Headstage
- Input Impedance: 1 x 1010 Ω shunted by 6 pF
- Input Voltage: ±1.5 V maximum
- Common Mode Voltage: ±13 V maximum
- Common Mode Rejection: 100 dB at 60 Hz
- Leakage Current: 20 pA maximum
- Offset Voltage Range: ±120 mV
Voltage Clamp Ranges
- Int. Clamp Potentiometer: ±100 mV with 10-turn control
- Ext. Command: ±1 V
- Ext. Command Factor: 1 mV/10 mV applied
Current Clamp Ranges
- Clamp Potentiometer: ±1 mA
- External Command: ±10 mA
- Command Factor: 1 µA/10 mV applied
- Speed: 10 µs measured with model membrane
Resistance
- Fluid Resistance: 0-100 Ω standard
- Compensation Range: 0-1 kΩ optional
- Membrane Resistance Measurement: made with 2 Hz bipolar constant current square wave
- Ranges: 0-2 kΩ, injected current = 10 nA; 0-200 kΩ, injected current = 1 nA
- Membrane Resistance: 0-2 kΩ, 1mV/Ω
- Output (Dual Channel Epithelial Voltage Clamp): 0-200 kΩ, 10mV/Ω
- Panel Meter (Single and Dual Channel Epithelial Voltage Clamps): 3-1/2 digit LCD; Voltage Range: 200 mV max; Current Range: 2000 µA max
Outputs
- Voltage Monitor: x10
- Current Monitor: 10 mV/µA
Compliance
- Single Channel Epithelial Voltage Clamp: ±120 V
- Dual Channel Epithelial Voltage Clamp: ±50 V
Timers (A & B) Dual Channel Epithelial Voltage Clamp
- Range: 10 ms to 1000 s, set with 2 digit resolution and 4 ranges (each channel)
- Power Requirements: 100-130 VAC or 220-240 VAC, 50/60 Hz, 15 VA
Physical Dimensions, H x W x D, and Weight
- Single Channel Epithelial Voltage Clamp Amplifier: 8.9 x 43.2 x 30.5 cm; 6.4 kg
- Dual Channel Epithelial Voltage Clamp Amplifier: 8.9 x 43.2 x 30.5 cm; 9.1 kg
- Headstage: 7.7 x 7.7 x 5 cm
